iLite® ADCC Activity Assay 
Product Line

The iLite ADCC Activity Assay product line, is a reporter gene system which is unique in its set-up.

The product line includes ADCC Effector Assay Ready Cells and various Target Assay Ready Cells, engineered to work optimally together and give unparalleled sensitivity. 


Each iLite ADCC Activity Assay includes Effector and Target cells as well as a negative control cell line for each specific target. The control cell line allows screening for unspecific activation of ADCC, giving added value to each experiment.

As the majority of the iLite cell lines, the iLite ADCC Effector (V) Assay Ready Cells also have a secondary luciferase readout, from a luciferase expressed under the control of a constitutive promotor. This enables normalization of each individual readout according to cell number and thereby accounts for any potential matrix effects.

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Figure 1. The interaction between the reporter gene effector cell and the target cell, generated by the crosslinking of the two cells via a specific drug antibody. The resulting luminescence originates exclusively from this crosslinking and the signaling from the CD16 receptor to the Firefly Luciferase promoter. The strength of the luminescence correlates to the drugs ability to induce ADCC.

All components of the ADCC Activity Assays are available individually, and the Effector cells can be used with any iLite Target cell, or a target cell line of your choice.

ADCC Activity Assays in the Drug Development Process

The activity of many antibody-based therapeutics is mediated in part by their ability to induce antibody-dependent cell-mediated cytotoxicity (ADCC) and thereby enhancing the body's own immune response towards dysfunctional cells. By analyzing the ADCC activity induced by different drugs or drug candidates, a measure of both drug potency and unwanted side effects can be provided.

As such, the iLite ADCC Activity Assay product line is a valuable tool for the whole drug development process, measuring the ability of a drug to induce ADCC activity in a straight forward, sensitive and reproducible manner. 

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Figure 2. When the target cell has been depleted for the specific surface target molecule, the crosslinking between the effector and the target cell cannot be established and hence no luminescence from Firefly Luciferase is generated. However, luminescence from the normalization gene NanoLuc, which is expressed under a constitutive promotor, is still present. 


    Key benefits of iLite® ADCC Activity Assays:

          - Unparalleled sensitivity
          - High serum tolerance
          - Normalization read-out included
          - Internal control available for screening of unspecific activity and reduction of background
          - Easy to use – no culturing required of target or effector cells

 

AVAILABLE - iLite® ADCC Activity Assay Product Line

 

Interested in trying iLite® ADCC Activity Assays?

Click here to contact us today!


 

 

COMING SOON - iLite® ADCC Activity Assay Product Line

 

Euro Diagnostica will continuously expand the iLite® ADCC Activity Assay product line with novel and higly specific target cells.

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news and upcoming launches - including
ADCC EGFR and mTNF-alpha target cells

 
In accepting delivery of iLite® Assay Ready Cells the recipient agrees not to sub-culture these cells, attempt to sub-culture them or to give them to a third party recipient, and only to use them directly in assays. Biomonitor iLite® cell-based products are covered by patents which are the property of Euro Diagnostica AB and any attempt to reproduce the delivered iLite® Assay Ready Cells is an infringement of these patents.

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