Rapid tests - lateral flow

Lateral flow tests are also called immunochromatographic strip (ICS) tests or simply strip-tests.

Lateral flow tests are a form of immunoassay in which the test sample flows along a solid substrate via capillary action. After the sample is applied to the test it encounters a coloured reagent, often latex or gold, The sample then continues to migrate across the membrane by capillary forces  until it reaches the capture zone where the target/conjugate complex will bind to the immobilised antibodies producing a visible line on the membrane. The sample then migrates further along the strip until it reaches the control zone, where excess conjugate will bind and produce a second visible line on the membrane. This control line indicates that the sample has migrated across the membrane as intended. Two clear lines on the membrane is a positive result. A single line in the control zone is a negative result.

Rapid tests - lateral flow