Jo-1 IgG EULISA

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Antibodies to the Jo-1 autoantigen (or histidyl-tRNA synthetase) are found in approximately 25% of polymyositis patients. Also a high proportion of patients with interstitial lung disease, associated with polymyositis, are positive for this autoantibody.

Product code
213796
Format
ELISA
Tests
Break apart micro titration strips (12x8) 96 wells
Calculation
Qualitative and quantitative
Antigen
Jo-1
Units
U/mL
Calibrators
6
Range
0-100 U/mL
Incubation time
30+30+30 min
Detection system
HRP/450 nm
Availability
CE marked. Not for sale in US

Intended use

Enzyme immunoassay for the detection of autoantibodies IgG against Jo-1.

Background

Polymyositis (PM) and dermatomyositis (DM) are inflammatory, autoimmune mediated disorders of the connective tissue with unknown etiology. They primarily damage skin and/or muscles, but may also affect other organs as e. g. the lung. Without treatment (standard therapy is immune suppression), they tend to develop into a life-threatening state. Since immunosuppressive agents are known to cause considerable side effects, early diagnosis of PM/DM is essential, in order to keep their dosage as low as possible.

A striking feature of PM/DM is the occurrence of antibodies to aminoacyl-tRNA synthetases; functionally related but immunologically distinct enzymes. About 30 % of all myositis patients carry antibodies against histidyl-tRNA synthetase, a 100 kDa dimeric antigen, located in the cytoplasma and known as Jo-1.

Technical information

The EULISA Jo-1 IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against Jo-1. The antigen used is a highly purified preparation of human histidyl-tRNA synthetase, expressed by baculovirus infected insect cells. The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.

Kit components and storage of reagents

Contents of the kit

  1. 1 microwell plate, coated with Jo-1 antigen and hermetically packed in a foil laminate pouch together with a desiccant bag.
  1. Sample buffer, 100 mL, ready-to-use, orange coloured.
  1. Wash buffer, 100 mL, 10 x-concentrate, blue coloured.
  1. 6 calibrators, 2.0 mL each 0, 1.0, 3.0, 10, 30 and 100 U Jo-1 antibodies (IgG) / mL ready-to-use, gradually blue coloured.
  1. Negative and positive control, 2.0 mL each, ready-to-use, green and red coloured, respectively.
  1. Anti-human IgG HRP conjugate, 14 mL, ready-to-use, red coloured.
  1. Substrate solution, 14 mL, ready-to-use, colourless.
  1. Stop solution (0.5 M H2SO4), 14 mL, colourless, ready-to-use.
  1. Directions for use
  1. Lot-specific certificate of analysis

All kit components are stored at 2 - 8°C.