TG IgG EULISA

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High titers of autoantibodies to TG are frequently found in patients with goitrous thyroiditis (Hashimoto's disease), atrophic thyroiditis (myxedema) and Graves' disease (immunogenic form of hyperthyroidism). In most cases, TG antibodies are found together with TPO antibodies. TG antibodies are considered as better marker of thyroiditis induction while TPO antibodies more specifically indicate active thyroid inflammation.

Product code
211996
Format
ELISA
Tests
Break apart micro titration strips (12x8) 96 wells
Calculation
Qualitative and quantitative
Antigen
Thyroglobulin (TG)
Units
IU/mL
Calibrators
6
Range
0-3 000 IU/mL serum
Incubation time
30+30+30 min
Detection system
HRP/450 nm
Availability
CE marked. Not for sale in US

Intended use

Enzyme immunoassay for the detection of autoantibodies IgG against TG.

Background

Autoimmune-mediated, pathogenically related disorders of the thyroid gland are characterized by the occurrence of autoantibodies directed at thyroid antigens, such as thyroglobulin (TG), thyroid peroxidase (TPO) and TSH receptor.

TG is a 660 kDa dimeric globular glycoprotein that is involved in the synthesis and storage of thyroid hormones. It constitutes the major component of the central lumen of the thyroid gland and one of its predominant autoantigens.

High titers of autoantibodies to TG are frequently found in patients with goitrous thyroiditis (Hashimoto's disease), atrophic thyroiditis (myxedema) and Graves' disease (immunogenic form of hyperthyroidism). However, their concentration does not usually correlate with the degree of thyroid dysfunction.

In most cases, TG antibodies are found together with TPO antibodies. TG antibodies are considered as better marker of thyroiditis induction while TPO antibodies more specifically indicate active thyroid inflammation. At a low percentage, TG antibodies also occur in the normal population and their prevalence is increasing with age.

Technical information

The EULISA TG IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against TG. The immobilised antigen is a highly purified preparation of human TG.

The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.

Kit components and storage of reagents

Contents of the kit

  1. 1 microwell plate, coated with TG and hermetically packed in a foil laminate pouch together with a desiccant bag.
  1. Sample buffer, 100 mL, ready-to-use, orange coloured.
  1. Wash buffer, 100 mL, 10 x-concentrate, blue coloured.
  1. 6 calibrators, 2.0 mL each, 0, 30, 100, 300, 1 000 and 3 000 IU TG antibodies (IgG)/mL ready-to-use, gradually blue coloured.
  1. Negative and positive control, 2.0 mL each, ready-to-use, green and red coloured, respectively.
  1. Anti-human IgG HRP conjugate, 14 mL, ready-to-use, red coloured.
  1. Substrate solution, 14 mL, ready-to-use, colourless.
  1. Stop solution (0.5 M H2SO4), 14 mL, colourless, ready-to-use.
  1. Directions for use
  1. Lot-specific certificate of analysis

All kit components are stored at 2 - 8°C.