Enzyme immunoassay for the detection of autoantibodies IgG against TG.
Autoimmune-mediated, pathogenically related disorders of the thyroid gland are characterized by the occurrence of autoantibodies directed at thyroid antigens, such as thyroglobulin (TG), thyroid peroxidase (TPO) and TSH receptor.
TG is a 660 kDa dimeric globular glycoprotein that is involved in the synthesis and storage of thyroid hormones. It constitutes the major component of the central lumen of the thyroid gland and one of its predominant autoantigens.
High titers of autoantibodies to TG are frequently found in patients with goitrous thyroiditis (Hashimoto's disease), atrophic thyroiditis (myxedema) and Graves' disease (immunogenic form of hyperthyroidism). However, their concentration does not usually correlate with the degree of thyroid dysfunction.
In most cases, TG antibodies are found together with TPO antibodies. TG antibodies are considered as better marker of thyroiditis induction while TPO antibodies more specifically indicate active thyroid inflammation. At a low percentage, TG antibodies also occur in the normal population and their prevalence is increasing with age.
The EULISA TG IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against TG. The immobilised antigen is a highly purified preparation of human TG.
The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.
Contents of the kit
All kit components are stored at 2 - 8°C.