Enzyme immunoassay for the detection of autoantibodies IgG against TPO.
Autoimmune-mediated, pathogenically related disorders of the thyroid gland are characterized by the occurrence of autoantibodies directed at thyroid antigens, such as thyroglobulin (TG), thyroid peroxidase (TPO) and TSH receptor.
TPO (formerly known as thyroid microsomal antigen) is a thyrocyte-specific, glycosylated 105 kDa hemoprotein, located in the microsome membrane and involved in the synthesis of thyroid hormones. It constitutes a major antigen of the thyroid gland.
High titers of autoantibodies to TPO are frequently found in patients with goitrous thyroiditis (Hashimoto's disease), atrophic thyroiditis (myxedema) and Graves' disease (immunogenic form of hyperthyroidism). However, the antibody concentration does not usually reflect the degree of thyroid dysfunction or therapy effectivity. TPO inhibition caused by autoantibodies is believed to decrease the synthesis of thyroid hormones, thereby leading to hypothyroidism.
In most cases, TPO antibodies are found together with TG antibodies. TG antibodies are considered as better marker of thyroiditis induction while TPO antibodies more specifically indicate active thyroid inflammation. At a low percentage, TPO antibodies also occur in the normal population with a clear female predominance, their prevalence increasing with age.
The EULISA TPO IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against TPO. The immobilised antigen is a highly purified preparation of recombinant human TPO. The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.
Contents of the kit
All kit components are stored at 2 - 8°C.