CENP-B IgG EULISA

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Anti-centromere antibodies (ACAs) have been identified as valuable diagnostic markers in patients with CREST syndrome. For this syndrome, the ACAs recognise a family of proteins that remain in the centromere region of eukaryotic cells throughout the cell cycle. Among the main antigenic proteins (called CENP-A, CENP-B and CENP-C), CENP-B is especially useful since almost all sera with ACA recognise a certain carboxy-terminal fragment of this protein.

Product code
213696
Format
ELISA
Tests
Break apart micro titration strips (12x8) 96 wells
Calculation
Qualitative and quantitative
Antigen
CENP-B
Units
U/mL
Calibrators
6
Range
0-60 U/mL
Incubation time
30+30+30 min
Detection system
HRP/450 nm
Availability
CE marked. Not for sale in US

Intended use

Enzyme immunoassay for the detection of autoantibodies IgG against CENP-B.

Background

Patients with CREST syndrome, one form of Progressive Systemic Sclerosis (PSS, scleroderma) rarely carry Scl-70 autoantibodies. For this syndrome, autoantibodies directed at antigens of the chromosome centromere (ACA) have been identified as valuable diagnostic marker. They recognise a family of proteins that remain in the centromere region of eukaryotic cells throughout the cell cycle. Among the main antigenic proteins (called CENP-A, CENP-B and CENP-C), CENP-B is especially useful since almost all sera with ACA recognise a certain carboxy-terminal fragment of this protein.

Technical information

The EULISA CENP-B IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against CENP-B. The antigen used is a highly purified preparation of recombinant human CENP-B, expressed in baculovirus-infected insect cells. The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.

Kit components and storage of reagents

Contents of the kit

  1. 1 microwell plate, coated with CENP-B and hermetically packed in a foil laminate pouch together with a desiccant bag.
  1. Sample buffer, 100 mL, ready-to-use, orange coloured.
  1. Wash buffer, 100 mL, 10 x-concentrate, blue coloured.
  1. 6 calibrators, 2.0 mL each, 0, 0.60, 2.0, 6.0, 20 and 60 U CENP-B antibodies (IgG)/mL, ready-to-use, gradually blue coloured.
  1. Negative and positive control, 2.0 mL each, ready-to-use, green and red coloured, respectively.
  1. Anti-human IgG HRP conjugate, 14 mL, ready-to-use, red coloured.
  1. Substrate solution, 14 mL, ready-to-use, colourless.
  1. Stop solution (0.5 M H2SO4), 14 mL, colourless, ready-to-use.
  1. Directions for use
  1. Lot-specific certificate of analysis

All kit components are stored at 2 - 8°C.