Enzyme immunoassay for the detection of autoantibodies IgG against CENP-B.
Patients with CREST syndrome, one form of Progressive Systemic Sclerosis (PSS, scleroderma) rarely carry Scl-70 autoantibodies. For this syndrome, autoantibodies directed at antigens of the chromosome centromere (ACA) have been identified as valuable diagnostic marker. They recognise a family of proteins that remain in the centromere region of eukaryotic cells throughout the cell cycle. Among the main antigenic proteins (called CENP-A, CENP-B and CENP-C), CENP-B is especially useful since almost all sera with ACA recognise a certain carboxy-terminal fragment of this protein.
The EULISA CENP-B IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against CENP-B. The antigen used is a highly purified preparation of recombinant human CENP-B, expressed in baculovirus-infected insect cells. The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.
Contents of the kit
All kit components are stored at 2 - 8°C.