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Measurement of MPO-specific ANCA (Anti-neutrophil cytoplasmic antibodies) is an important adjunct to clinical findings in the evaluation of clinical sub types within the systemic vasculitides spectrum. ANCAs are directed against cytoplasmic components of neutrophil granulocytes and monocytes.

Product code
Break apart micro titration strips (12x8) 96 wells
Qualitative and quantitative
MPO (myeloperoxidase)
0-100 U/mL
Incubation time
30+30+30 min
Detection system
HRP/450 nm
CE marked. Not for sale in US

Intended use

Enzyme immunoassay for the detection of autoantibodies IgG against MPO.


Anti-neutrophil cytoplasmic antibodies (ANCA) are directed against cytoplasmic components of neutrophil granulocytes and monocytes. They have proven to be useful serological markers for a number of systemic, autoimmune mediated vasculitides.

MPO autoantibodies are most closely associated with microscopic polyangiitis. They also occur in patients with polyangiitis overlap syndrome without renal involvement, idiopathic or vasculitis-associated crescentic glomerulonephritis, classic polyarteritis nodosa and Churg-Strauss syndrome. The titer of MPO antibodies is considered to reflect flares and remissions of these diseases in individual patients.

Technical information

The EULISA MPO IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against MPO. The immobilised antigen is a highly purified preparation of MPO, isolated from human granulocytes. The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.

Kit components and storage of reagents

Contents of the kit

  1. 1 microwell plate, coated with MPO and hermetically packed in a foil laminate pouch together with a desiccant bag.
  1. Sample buffer, 100 mL, ready-to-use, orange coloured.
  1. Wash buffer, 100 mL, 10 x-concentrate, blue coloured.
  1. 6 calibrators, 2.0 mL each 0, 3.0, 8.0, 18, 45 and 100 U MPO antibodies (IgG)/mL ready-to-use, gradually blue coloured.
  1. Negative and positive control, 2.0 mL each, ready-to-use, green and red coloured, respectively.
  1. Anti-human IgG HRP conjugate, 14 mL, ready-to-use, red coloured.
  1. Substrate solution, 14 mL, ready-to-use, colourless.
  1. Stop solution (0.5 M H2SO4), 14 mL, colourless, ready-to-use.
  1. Directions for use
  1. Lot-specific certificate of analysis

All kit components are stored at 2 - 8°C.