Anti-Jo-1 DIASTAT® - Discontinued

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Antibodies to the Jo-1 autoantigen, also recognised as the enzyme histidyl-tRNA synthetase, are found in approximately 25% of polymyositis patients. A high proportion of patients with interstitial lung disease, associated with polymyositis, are also positive for this autoantibody.

Product code
FAJO 200
Format
ELISA
Tests
96 wells
Calculation
Qualitative
Antigen
Purified Jo-1 antigen
Calibrators
Ratio
Incubation time
60+30+30 min
Detection system
ALP/PMP (550 nm)
Availability
CE marked. For sale in US

Intended use

The DIASTAT® anti-Jo-1 test is a qualitative enzyme-linked immunosorbent assay (ELISA) for the detection of autoantibodies specific for Jo-1 antigen in human serum or plasma. It is intended to aid in the diagnosis of polymyositis and is not definitive in isolation. Autoantibody levels represent one parameter in a multicriterion diagnostic process.

 

Background

Systemic rheumatic diseases are autoimmune disorders such as systemic lupus erythematosus, polymyositis, Sjögren's syndrome, scleroderma and rheumatoid arthritis. A general feature of systemic rheumatic diseases is the presence of circulating antibodies to a variety of cellular antigens. The detection and serological characterisation of specific autoantibodies plays an important role in the differential diagnosis of these diseases.

Antibodies to the Jo-1 autoantigen, also recognised as the enzyme histidyl-t RNA synthetase, are found in approximately 25% of polymyositis patients. A high proportion of patients with interstitial lung disease, associated with polymyositis, are also positive for this autoantibody.

Technical information

The wells of the microtitre strips are coated with affinity-purified Jo-1 antigen. During the first incubation, specific autoantibodies in diluted serum or plasma bind to the antigen-coated surface. The wells are then washed to remove unbound components. In the second incubation, the Conjugate, enzyme-labelled antibodies to human IgG, binds any surface-bound autoantibodies. After further washing, specific autoantibodies are traced by incubation with the Substrate. Addition of Stop Solution terminates the reaction, resulting in a coloured end-product. The amount of Conjugate bound is measured in absorbance units. The amount of Conjugate bound by the sample is compared with that bound by the Reference Control.