GBM IgG EULISA - Discontinued

Add to my product list
The EULISA GBM IgG is used for detection of IgG autoantibodies to glomerular basement membrane (GBM). GBM autoantibodies are commonly found in patients with Goodpasture's syndrome, a serious disease characterized by lung haemorrhage and renal failure. As there are several other autoimmune diseases which may present with similar symptoms, this kit is a useful aid in differentiation between these diseases.

Product code
Break apart micro titration strips (12x8) 96 wells
Qualitative and quantitative
GBM (glomerular basement membrane)
0-100 U/mL
Incubation time
30+30+30 min
Detection system
HRP/450 nm
CE marked. Not for sale in US

Intended use

Enzyme immunoassay for the detection of autoantibodies IgG against GBM.


In patients suffering from Goodpasture's syndrome GBM-specific autoantibodies, in co-operation with the complement, initiate an inflammatory destruction of the basement membrane of kidney’s glomeruli and lung alveolus. The titers of these autoantibodies are of prognostic importance. In patients with renal disease, GBM-specific autoantibodies should always be determined together with anti-neutrophil cytoplasmic antibodies (ANCA) since ANCA-associated glomerulonephritis requires a treatment different from that of GS.

Technical information

The EULISA GBM IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against GBM. The immobilised autoantigen is the non-collagenous domain 1 (NC1) of the alpha3-chain of human collagen type IV (a3(IV)), a recombinant preparation expressed in the baculovirus system and purified by an affinity / ion exchange chromatographic procedure. The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.

Kit components and storage of reagents

Contents of the kit

  1. 1 microwell plate, coated with GBM and hermetically packed in a foil laminate pouch together with a desiccant bag.
  1. Sample buffer, 100 mL, ready-to-use, orange coloured.
  1. Wash buffer, 100 mL, 10 x-concentrate, blue coloured.
  1. 6 calibrators, 2.0 mL each 0, 3.0, 8.0, 18, 45 and 100 U GBM antibodies (IgG)/mL ready-to-use, gradually blue coloured.
  1. Negative and positive control, 2.0 mL each, ready-to-use, green and red coloured, respectively.
  1. Anti-human IgG HRP conjugate, 14 mL, ready-to-use, red coloured.
  1. Substrate solution, 14 mL, ready-to-use, colourless.
  1. Stop solution (0.5 M H2SO4), 14 mL, colourless, ready-to-use.
  1. Directions for use
  1. Lot-specific certificate of analysis

All kit components are stored at 2 - 8°C.