Enzyme immunoassay for the detection of autoantibodies IgG against GBM.
In patients suffering from Goodpasture's syndrome GBM-specific autoantibodies, in co-operation with the complement, initiate an inflammatory destruction of the basement membrane of kidney’s glomeruli and lung alveolus. The titers of these autoantibodies are of prognostic importance. In patients with renal disease, GBM-specific autoantibodies should always be determined together with anti-neutrophil cytoplasmic antibodies (ANCA) since ANCA-associated glomerulonephritis requires a treatment different from that of GS.
The EULISA GBM IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against GBM. The immobilised autoantigen is the non-collagenous domain 1 (NC1) of the alpha3-chain of human collagen type IV (a3(IV)), a recombinant preparation expressed in the baculovirus system and purified by an affinity / ion exchange chromatographic procedure. The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.
Contents of the kit
All kit components are stored at 2 - 8°C.