iLite® VEGF Assay Ready Cells

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iLite® VEGF Assay Ready Cells is a human embryonic kidney cell line (HEK-293*; ATCC #CRL-1573) that have been genetically engineered and optimized to respond to VEGF with specific, proportional expression of Firefly Luciferase. 

* The HEK-293 cell line has been used under a license obtained from AdVec Inc.

Product code
BM4021
Format
Assay Ready Cells
Calculation
Quantitative
Antigen
VEGF
Detection system
Luminescence
Availability
Research use only

The iLite® VEGF Assay Ready Cells can be used for the quantification of VEGF activity, VEGF inhibitor activity and for determination of neutralizing antibodies against VEGF inhibitors in human serum. 

Vascular Endothelial Growth Factor (VEGF) is a signalling protein which is involved in both normal vascular growth and pathological angiogenesis. Without angiogenesis, growth of solid tumours would be limited by oxygen supply. Tumours which express VEGF can overcome this limitation and are thus able to grow and metastasize. For this reason, different anti-cancer therapies targeting VEGF have emerged, e.g. a humanized anti-VEGF antibody such as bevacizumab (AvastinTM, Genentech) is currently widely used as a first-line therapy for colorectal cancer (1,2). 

>250µl of Assay Ready Cells suspended in DMEM medium with 20% heat inactivated fetal bovine serum (FBS) and 10% dimethyl sulfoxide (DMSO).