iLite® GM-CSF Assay Ready Cells

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iLite® GM-CSF Assay Ready Cells are a genetically engineered reporter gene cell line (U937, ATCC# CRL1593.2) responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Product code
BM4050
Format
Assay Ready Cells
Calculation
Quantitative
Antigen
GM-CSF
Detection system
Luminescence
Availability
Research use only

The iLite® GM-CSF Assay Ready Cells can be used for the quantification GM-CSF activity, GM-CSF inhibitor activity, and for determination of neutralizing antibodies against such either in buffer systems or human serum. 
• Quantification of GM-CSF activity using iLite® GM-CSF Assay Ready Cells (E-220-GB) 
• Determination of GM-CSF neutralizing activity using iLite® GM-CSF Assay Ready Cells (E-221-GB)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine which stimulates the production of granulocytes and monocytes from bone marrow precursors (1). As such, it serves as an important key in both humoral and cell mediated immunity. Recombinant GM-CSF has several therapeutic uses; in order to accelerate leukocyte recovery after bone marrow transplantation, to replenish leukocytes after chemotherapy and for treatment of fungal infections. 
 
The immunostimulatory effects of GM-CSF have also been used in oncolytic viruses modified to include genes coding for GM-CSF, thus enhancing recruitment of the immune response to tumor cells. In addition, the discovery of a pro-inflammatory role of GM-CSF in autoimmune disease has led to development of several GM-CSF inhibitor drugs (2, 3).
 

>250 µL of iLite® Assay Ready Cells suspended in RPMI 1640 with 20% FBS, mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).