iLite® ADCC Target EGFR (-) Assay Ready Cells

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iLite® ADCC Target EGFR (-) Assay Ready Cells are based on a human embryonic kidney cell line, HEK293 (ATCC# CRL-1573), and have been genetically engineered to and optimized to be depleted of EGRF expression. The cells are for use as negative control  together with iLite ADCC Effector (V) Assay Ready Cells and iLite® ADCC Target EGFR (+) Assay Ready Cells for measuring the ADCC activity of anti-EGFR antibodies.

Product code
Detection system
Research Use Only (RUO)

The iLite® anti-ADCC Target EGFR (-) Assay Ready Cells can be used together with iLite® ADCC Effector (V) and iLite® ADCC Target EGFR (+) Assay Ready Cells for the quantification ADCC activity.

  • Quantification of anti-EGFR ADCC activity (LABEL-DOC-0407)

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism whereby pathogenic cells are lysed by lymphocytes, most often Natural Killer (NK) cells. The mechanism involves binding of antibodies to surface antigens on the pathogen. Crosslinking of these antibodies to NK cells through the binding of the Fc-portion to Fc receptors on the NK cells leads to activation of the NK cell and formation of an immune synapse with the pathogenic cell. The NK cell releases cytotoxic granules containing granzymes and perforin into the synapse, leading to apoptosis of the targeted cell (1). 

>250 µL of Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010).