iLite® anti-mTNF-alpha ADCC Activity Set

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This product contains a packaged set of the three individual products, one vial of each:
iLite® ADCC Effector (V) Assay Ready Cells (BM5001), 
iLite® ADCC Target mTNF-alpha (+) Assay Ready Cells (BM5013) 
iLite® ADCC Target mTNF-alpha (-) Assay Ready Cells (BM5014)
 
All three products are genetically engineered human cells to be used for measuring the ADCC activity of anti-mTNF-alpha antibodies. For more information about the individual cell lines, please consult the specific Product Specifications on www.svarlifescience.com.

Product code
BM5095
Format
Assay Ready Cells

The products included in the iLite® anti-mTNF-alpha ADCC Activity Set can be used together for the quantification ADCC activity of anti-mTNF-alpha antibodies. 
 

 Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism whereby pathogenic cells are lysed by lymphocytes, most often Natural Killer (NK) cells. The mechanism involves binding of antibodies to surface antigens on the pathogen. Crosslinking of these antibodies to NK cells through the binding of the Fc-portion to Fc receptors on the NK cells leads to activation of the NK cell and formation of an immune synapse with the pathogenic cell. The NK cell releases cytotoxic granules containing granzymes and perforin into the synapse, leading to apoptosis of the targeted cell (1).

Monoclonal anti-TNF-alpha inhibitors are one of the most commons types of antibody drugs on the market. The mechanism by which these drugs act is most often blocking of soluble TNF-alpha or the TNF receptor. Since TNF-alpha is produced through cleavage of a membrane bound form of the protein, there is a risk that anti-TNF-alpha antibodies bind the membrane bound TNF-alpha and trigger effector functions, such as ADCC. By using the iLite ADCC mTNF-alpha Activity Assay, the risk of an anti-TNF-alpha antibody drug inducing ADCC can be quantified.

3 vials of iLite® Assay Ready Cells (>250 µL). For contents of the individual vials, please please consult the specific Product Specifications.