iLite® G-CSF Assay Ready Cells

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iLite® G-CSF Assay Ready Cells are avian DT-40 cells (ATCC#CRL2111) which have been genetically engineered to be responsive to G-CSF, resulting in the specific expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.

 

Product code
BM4055
Format
Assay Ready Cells
Calculation
Quantitative
Antigen
G-CSF
Detection system
Luminescence
Availability
Research use only

The iLite® G-CSF Assay Ready Cells can be used for the quantification G-CSF activity and G-CSF inhibitor activity, either in buffer systems or human serum. 

Application Notes for the following assays are available:

  • Quantification of functional G-CSF 
  • Quantification of G-CSF inhibitor activity

 

Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates cells in the bone marrow to differentiate into granulocytes which are subsequently release into the blood. The G-CSF-Receptor is present on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates proliferation and differentiation into mature blood cells. Different types of pharmaceutical analogs are used as a therapeutic agent to stimulate the bone marrow to produce more neutrophils (white blood cells) to fight infection in patients undergoing chemotherapy for cancer (1,2).

>250 µL of Assay Ready Cells suspended in cryoprotective medium from Amsbio (Cat. No 11888).